Overview

Methadone hydrochloride, also known as (±)-6-(dimethylamino)-4,4-diphenyl-3-heptanone hydrochloride, is a synthetic opioid belonging to the diphenylmethane class of compounds . This product is supplied as a high-purity certified reference material (CRM) for use in forensic and clinical toxicology laboratories . As a Schedule II controlled substance in the United States, it serves as an essential reference standard for the identification and quantification of methadone and its primary metabolite, EDDP (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine), in seized materials and biological specimens .

Chemical & Physical Properties

The material typically presents as a white to off-white crystalline powder. For analytical preparation, stock solutions are commonly prepared in methanol or acetonitrile for LC-MS/MS and GC-MS workflows. The compound exhibits a melting point of approximately 235°C and is characterized by a tertiary amine functional group and two phenyl rings .

Primary Research Applications

Methadone hydrochloride is primarily utilized as a certified reference material for the identification and quantification of methadone and its metabolite EDDP in forensic toxicology and clinical research . Its primary applications include method development and validation for LC-MS/MS and GC-MS, as well as confirmatory analysis of biological specimens in urine drug testing and forensic casework . The compound also serves as a standard for pharmacokinetic studies and multi-residue analysis of opioids in blood and serum samples . Certified reference materials are rigorously tested under ISO/IEC 17025 and ISO 17034 accreditation and are available as DEA-exempt preparations for streamlined laboratory ordering .

Storage & Stability

For optimal stability, the product should be stored in a freezer in a tightly sealed container. Certified reference material solutions in methanol or acetonitrile should be handled promptly after opening to maintain accurate concentration. Researchers must adhere to standard safe handling practices for hazardous chemicals and maintain appropriate security for controlled substances.

Closing Statement

This high-purity certified reference material is intended exclusively for in vitro laboratory use in forensic and analytical research. Researchers should consult the Certificate of Analysis (COA) for specific lot data and certified purity values. All handling must be performed within a properly controlled professional laboratory environment, and users must ensure compliance with all local and federal regulations regarding controlled substances.

⚠️ DISCLAIMER:
This product is sold strictly for laboratory and research purposes only. It is NOT intended for human or veterinary consumption, diagnostic use, or any application involving living organisms. All customers must be qualified and trained laboratory professionals. Buyer assumes all responsibility for safe handling, storage, and legal compliance in accordance with their local, state, and federal regulations. Always use appropriate personal protective equipment (PPE) and work within a properly ventilated fume hood.